What is the C18 and C8 column ?
C18 and C8 columns are types of stationary phases commonly used in chromatography, particularly in high-performance liquid chromatography (HPLC).
These columns are named after the length of their alkyl chains. The "C" stands for "carbon," and the number indicates the number of carbon atoms in the alkyl chain. For example:
- C18 column has an octadecyl (18 carbons) stationary phase.
- C8 column has an octyl (8 carbons) stationary phase.
The choice between C18 and C8 columns depends on the specific separation requirements of the compounds being analyzed. C18 columns are typically more hydrophobic and offer stronger retention for nonpolar compounds, while C8 columns offer intermediate polarity and are suitable for separating moderately polar compounds.